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Image Search Results
Journal: eLife
Article Title: 3D virtual histopathology of cardiac tissue from Covid-19 patients based on phase-contrast X-ray tomography
doi: 10.7554/eLife.71359
Figure Lengend Snippet: ( A ) HE stain of a 3-m-thick paraffin section of one sample from a patient who died from Covid-19 (Cov-I, Scalebar: 100 μ m ). In total, 26 postmortem heart tissue samples were investigated: 11 from Covid-19 patients, 4 from influenza patients, 5 from patients who died with myocarditis and six control samples. ( B ) From each of the samples, a biopsy punch with a diameter of 3.5 mm was taken and transferred onto a holder for the tomography acquisition. After tomographic scans of all samples at the laboratory setup, Covid-19 and control specimens were investigated at the synchrotron. Furthermore,at the laboratory and parallel beam setup at the synchrotron, one punch with a diameter of 1 mm was taken from one of the control and Covid-19 samples for investigations at high resolution. ( C ) Sketch of the laboratory micro-CT setup. Tomographic scans of all samples were recorded in cone beam geometry with an effective pixel size of p x eff = 2 μ m using a liquid metal jet source (EXCILLUM, Sweden). ( D ) Sketch of the parallel beam setup of the GINIX endstation (P10 beamline, DESY, Hamburg). In this geometry, datasets of Covid-19 and control samples were acquired at an effective voxel size of 650 nm 3 . One plane of each sample was covered by 3×3 tomographic recordings. For each sample a plane of 3×3 tomographic acquisitions was recorded. ( E ) Cone beam setup of the GINIX endstation. After the investigation in parallel geometry, the 1 mm biopsy punches of one control and Covid-19 sample were probed and a high resolution scan in cone beam geometry was recorded. This configuration is based on a coherent illumination by a wave guide and allows for high geometric magnification and effective voxel sizes below 200 nm .
Article Snippet: X-rays emitted from a
Techniques: H&E Stain, Paraffin Section, Tomography, Micro-CT