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Image Search Results
Journal: bioRxiv
Article Title: 3D virtual Histopathology of Cardiac Tissue from Covid-19 Patients based on Phase-Contrast X-ray Tomography
doi: 10.1101/2021.09.16.460594
Figure Lengend Snippet: (A) HE stain of a 3 μm thick paraffin section of one sample from a patient who died from Covid-19 (Cov-I, Scalebar: 100 μm). In total, 26 post mortem heart tissue samples were investigated: 11 from Covid-19 patients, 4 from influenza patients, 5 from patients who died with myocarditis and 6 control samples. (B) From each of the samples a biopsy punch with a diameter of 3.5mm was taken and transferred onto a holder for the tomography acquisition. After tomographic scans at the laboratory and parallel beam setup at the synchrotron, one punch with a diameter of 1mm was taken from one of the control and Covid-19 samples. (C) Sketch of the laboratory micro-CT setup. Tomographic scans of all samples were recorded in cone beam geometry with an effective pixel size of px eff = 2 μm using a liquid metal jet source (EXCILLUM, Sweden). (D) Sketch of the parallel beam configuration GINIX endstation (P10 beamline, DESY, Hamburg). In this geometry, datasets of Covid-19 and control samples were acquired at an effective voxel size of 650 nm 3 . For each sample a plane of 3×3 tomographic acquisitions was recorded. (E) Cone beam configuration of the GINIX setup. After the investigation in parallel geometry, a biopsy with a diameter of 1mm was taken from a control sample and a high resolution scan in cone beam geometry was recorded. This configuration is based on a coherent illumination by a wave guide and allows for high geometric magnification and effective voxel sizes below 200 nm.
Article Snippet: X-rays emitted from a
Techniques: H&E Stain, Paraffin Section, Tomography, Micro-CT